Journal: BioMed Research International
Article Title: Autophagy and Human Parturition: Evaluation of LC3 Expression in Placenta from Spontaneous or Medically Induced Onset of Labor
doi: 10.1155/2013/689768
Figure Lengend Snippet: LC3, CRF, and HIF-1 α expression. (a) for each antibody the spatial distribution of immunohistochemical staining was the same in cases with spontaneous and induced labor. (b) The analysis of data from optical density values of Western blotting bands shows no differences of LC3-II and HIF-1 α expression between groups. On the contrary CRF is higher in spontaneous labor. LC3-II, HIF-1 α , and CRF are normalized onto α -tubulin. (c) No correlation between LC3-II and CRF expression was found. SP: spontaneous labor; PG: induction with prostaglandin; PO: induction with prostaglandin and oxytocin; OX: induction with oxytocin.
Article Snippet: After washing, the blots were incubated with antirabbit horseradish peroxidase-conjugated secondary antibody (Jackson ImmunoResearch, dilution 1 : 10000). α -Tubulin was used for data normalization ( α -tubulin (E-19)-R antibody, Santa Cruz Biotechnology, dilution 1 : 1000).
Techniques: Expressing, Immunohistochemical staining, Staining, Western Blot